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Cloning and Expression
Enzymes
TaqI-v2
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TaqI-v2
TaqI-v2 from New England Biolabs
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Citations:
(10)
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New England Biolabs for
TaqI-v2
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Description
TaqI
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
TaqI-v2
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0149T
Concentration
100,000 units/ml
Quantity
4000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
5'-T/CGA-3'
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (10)
(1)
Suppression of inflammatory arthritis by the parasitic worm product ES-62 is associated with epigenetic changes in synovial fibroblasts
PLOS Pathogens
November 8, 2021
Marlene Corbet, Miguel A. Pineda, Kun Yang, Anuradha Tarafdar, Sarah McGrath, Rinako Nakagawa, Felicity E. Lumb, Colin J. Suckling, William Harnett, Margaret M. Harnett
(2)
Increased burden of mitochondrial DNA deletions and point mutations in early-onset age-related hearing loss in mitochondrial mutator mice
Experimental Gerontology
October 1, 2019
Mi-Jung Kim et al.
(
Thermo Fisher Scientific
,
B28
) and digested by adding 100 U of TaqI restriction enzyme (
New England Biolabs
,
R0149M
) every hour over a 5-h time span. The mtDNA was then cleaned up with the
Oligo Clean & Concentrator
(3)
Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
Nature Communications
December 1, 2018
Yeonsoo Yoon, Dan Wang, Phillip W. L. Tai, Joy Riley, Guangping Gao, Jaime A. Rivera-Pérez
control sample. Genomic material (20 μg total input) was fragmented by TaqαI digestion (
NEB
, Cat. No.
R0149M
). Fragmented DNAs were subjected to phenol–chloroform extraction and ethanol precipitation to purify
(4)
Multiple Molecular Mechanisms Rescue mtDNA Disease in C. elegans
Cell Reports
March 20, 2018
Suraiya Haroon et al.
performed whole-genome preps. 10–20 µg of total DNA was digested with TaqαI restriction enzyme (cat. no.
R0149T
,
New England Biolabs
) for 10 hr, with 1 µL of fresh enzyme added every hour. Digested DNA was amplified
(5)
Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryo
Wellcome Open Research
November 16, 2017
Alexander M. J. Eve, James C. Smith
(6)
Effects of calorie restriction on the lifespan and healthspan of POLG mitochondrial mutator mice
PLOS ONE
February 3, 2017
Shinichi Someya, Gregory C. Kujoth, Mi-Jung Kim, Timothy A. Hacker, Marc Vermulst, Richard Weindruch, Tomas A. Prolla
2017 2 / 11 Effects of calorie restriction on the lifespan of mitochondrial mutator
mice
(
NEB
,
R0149M
) every hour over a 5-hour time span. The mtDNA was then cleaned up with an oligo-concentrator kit from
(7)
Conservation and Divergence of Regulatory Strategies at Hox Loci and the Origin of Tetrapod Digits
PLoS Biology
January 21, 2014
Joost M. Woltering, Daan Noordermeer, Marion Leleu, Denis Duboule
enzyme was
DpnII
(New England Biolabs,
R0543M
), and the secondary enzyme was TaqαI (
New England Biolabs
,
R0149M
). In the latter case, DNA was cut for 8 h at 65°C. For the Tetraodon baits, assessed in transgenic
mice
,
(8)
25-hydroxyvitamin D deficiency, exacerbation frequency and human rhinovirus exacerbations in chronic obstructive pulmonary disease
BMC Pulmonary Medicine
December 1, 2012
Jennifer K Quint, Gavin C Donaldson, Nancy Wassef, John R Hurst, Michael Thomas, Jadwiga A Wedzicha
polymorphism (rs731236), a RFLP analysis was carried out by using the TaqIα restriction endonuclease enzyme (
R0149T
New England BioLabs
). The reaction consisted of; 2μl buffer, 0.2μl
BSA
, 7.7μl dH2O, 0.1μl RE (TaqIα)
(9)
Measuring the methylome in clinical samples: Improved processing of the Infinium Human Methylation450 BeadChip Array
Epigenetics
October 13, 2012
Hong Pan et al.
restriction enzyme digestion
for 2 h each by 150 U each of
MspI
and TaqαI (Catalog Nos.:
R0106S
and
R0149M
,
New England Biolabs
) at optimal temperatures, according to manufacturer’s instructions. The digested
(10)
A random mutation capture assay to detect genomic point mutations in mouse tissue
Nucleic Acids Research
June 1, 2011
Jocelyn H. Wright, Kristina L. Modjeski, Jason H. Bielas, Bradley D. Preston, Nelson Fausto, Lawrence A. Loeb, Jean S. Campbell
accurate. TaqI digestion of genomic DNA High concentration TaqαI (100 000 U/ml;
New England Biolabs
catalog#
R0149M
) is used to maximize enzyme concentration in the reaction. TaqαI is a mutant form of the enzyme that
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TaqI-v2 from New England Biolabs
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